electrode stx100c world precision instruments (World Precision Instruments)
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World Precision Instruments
electrode stx100c world precision instruments
Electrode Stx100c World Precision Instruments, supplied by World Precision Instruments, used in various techniques. Bioz Stars score: 94/100, based on 71 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/electrode+stx100c+world+precision+instruments/HTS+Electrodes+for+Use+with+the+EVOM2-STX100M/pmc07493029__jmcb___2019___0055_r3_supplementary_material_finalized_version_mjaa005-45-225-227
Average 94 stars, based on 71 article reviews
Electrode Stx100c World Precision Instruments, supplied by World Precision Instruments, used in various techniques. Bioz Stars score: 94/100, based on 71 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/electrode+stx100c+world+precision+instruments/HTS+Electrodes+for+Use+with+the+EVOM2-STX100M/pmc07493029__jmcb___2019___0055_r3_supplementary_material_finalized_version_mjaa005-45-225-227
Average 94 stars, based on 71 article reviews
electrode stx100c world precision instruments - by Bioz Stars,
2026-10
94/100 stars
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Membrane:Article Title: Cyclophilins A and B oppositely regulate renal tubular epithelial cell phenotype Article Snippet: Materials Product Reference Supplier Cells HK-2 #CRL-2190 ATCC, Manassas, VA, USA RPTEC/TERT1 #CRL-4031 ATCC, Manassas, VA, USA Compounds TGFβ #100-B-001 R&D Systems, Minneapolis, MN, USA MG-132 #BML-PI102 Enzo Life Sciences, Farmingdale, NY, USA cyclosporine-A #239835 Calbiochem, San Diego, CA, USA brefeldin-A #B6542 Sigma-Aldrich, St. Louis, MO, USA human CypB #NBC1-18424 Novus Biologicals, Littleton, CO, USA ionomycin #I0634 Sigma-Aldrich, St. Louis, MO, USA thapsigargin #586005 Calbiochem, San Diego, CA, USA Plates and inserts 24-well transwell plates with 0.4 μm pore polyester membrane inserts (HTS transwell-24 PET) #CLS3379-2EA Sigma-Aldrich, St. Louis, MO, USA Polycarbonate cell culture inserts with 8 μm pore size and 0.47 cm2 growth area (1×105 pores/cm2) #140629 NuncTM-ThermoFisher, Waltham, MA, USA silicone culture inserts #81176 Ibidi GmbH, Martinsried, Germany growth factor reduced (GFR)MatrigelTM Basement Membrane Matrix #356230 BD Biosciences, Franklin Lakes, NJ, USA Kits Lipofectamine 3000 transfection kit #L3000-001 ThermoFischer, Waltham, MA, USA High-Capacity RNA-to-cDNA kit #4387406 Applied Biosystems, Foster City, CA, USA TRIzol Reagent #15596-026 Ambion-Life Technolog., Carlsbad, CA, USA QuikChange II XL Site-Directed Mutagenesis Kit #200522 Agilent Technologies, Santa Clara, CA, USA Cell Proliferation Kit II (XTT) #11465015001 Roche, Mannheim, Germany Dual-Luciferase Reporter Assay System #E1910 Promega, Fitchburg, WI, USA CoIP anti-HA Affinity Matrix, clone 3F10 #11815016001 Roche, Mannheim, Germany HA peptide #11666975001 Roche, Mannheim, Germany Devices flow cytometer FACScalibur Becton Dickinson, Franklin Lakes, NJ, USA voltmeter Millicell-ERS Millipore, Burlington, MA, USA Positron Emission Tomography:Article Title: Cyclophilins A and B oppositely regulate renal tubular epithelial cell phenotype Article Snippet: Materials Product Reference Supplier Cells HK-2 #CRL-2190 ATCC, Manassas, VA, USA RPTEC/TERT1 #CRL-4031 ATCC, Manassas, VA, USA Compounds TGFβ #100-B-001 R&D Systems, Minneapolis, MN, USA MG-132 #BML-PI102 Enzo Life Sciences, Farmingdale, NY, USA cyclosporine-A #239835 Calbiochem, San Diego, CA, USA brefeldin-A #B6542 Sigma-Aldrich, St. Louis, MO, USA human CypB #NBC1-18424 Novus Biologicals, Littleton, CO, USA ionomycin #I0634 Sigma-Aldrich, St. Louis, MO, USA thapsigargin #586005 Calbiochem, San Diego, CA, USA Plates and inserts 24-well transwell plates with 0.4 μm pore polyester membrane inserts (HTS transwell-24 PET) #CLS3379-2EA Sigma-Aldrich, St. Louis, MO, USA Polycarbonate cell culture inserts with 8 μm pore size and 0.47 cm2 growth area (1×105 pores/cm2) #140629 NuncTM-ThermoFisher, Waltham, MA, USA silicone culture inserts #81176 Ibidi GmbH, Martinsried, Germany growth factor reduced (GFR)MatrigelTM Basement Membrane Matrix #356230 BD Biosciences, Franklin Lakes, NJ, USA Kits Lipofectamine 3000 transfection kit #L3000-001 ThermoFischer, Waltham, MA, USA High-Capacity RNA-to-cDNA kit #4387406 Applied Biosystems, Foster City, CA, USA TRIzol Reagent #15596-026 Ambion-Life Technolog., Carlsbad, CA, USA QuikChange II XL Site-Directed Mutagenesis Kit #200522 Agilent Technologies, Santa Clara, CA, USA Cell Proliferation Kit II (XTT) #11465015001 Roche, Mannheim, Germany Dual-Luciferase Reporter Assay System #E1910 Promega, Fitchburg, WI, USA CoIP anti-HA Affinity Matrix, clone 3F10 #11815016001 Roche, Mannheim, Germany HA peptide #11666975001 Roche, Mannheim, Germany Devices flow cytometer FACScalibur Becton Dickinson, Franklin Lakes, NJ, USA voltmeter Millicell-ERS Millipore, Burlington, MA, USA Cell Culture:Article Title: Cyclophilins A and B oppositely regulate renal tubular epithelial cell phenotype Article Snippet: Materials Product Reference Supplier Cells HK-2 #CRL-2190 ATCC, Manassas, VA, USA RPTEC/TERT1 #CRL-4031 ATCC, Manassas, VA, USA Compounds TGFβ #100-B-001 R&D Systems, Minneapolis, MN, USA MG-132 #BML-PI102 Enzo Life Sciences, Farmingdale, NY, USA cyclosporine-A #239835 Calbiochem, San Diego, CA, USA brefeldin-A #B6542 Sigma-Aldrich, St. Louis, MO, USA human CypB #NBC1-18424 Novus Biologicals, Littleton, CO, USA ionomycin #I0634 Sigma-Aldrich, St. Louis, MO, USA thapsigargin #586005 Calbiochem, San Diego, CA, USA Plates and inserts 24-well transwell plates with 0.4 μm pore polyester membrane inserts (HTS transwell-24 PET) #CLS3379-2EA Sigma-Aldrich, St. Louis, MO, USA Polycarbonate cell culture inserts with 8 μm pore size and 0.47 cm2 growth area (1×105 pores/cm2) #140629 NuncTM-ThermoFisher, Waltham, MA, USA silicone culture inserts #81176 Ibidi GmbH, Martinsried, Germany growth factor reduced (GFR)MatrigelTM Basement Membrane Matrix #356230 BD Biosciences, Franklin Lakes, NJ, USA Kits Lipofectamine 3000 transfection kit #L3000-001 ThermoFischer, Waltham, MA, USA High-Capacity RNA-to-cDNA kit #4387406 Applied Biosystems, Foster City, CA, USA TRIzol Reagent #15596-026 Ambion-Life Technolog., Carlsbad, CA, USA QuikChange II XL Site-Directed Mutagenesis Kit #200522 Agilent Technologies, Santa Clara, CA, USA Cell Proliferation Kit II (XTT) #11465015001 Roche, Mannheim, Germany Dual-Luciferase Reporter Assay System #E1910 Promega, Fitchburg, WI, USA CoIP anti-HA Affinity Matrix, clone 3F10 #11815016001 Roche, Mannheim, Germany HA peptide #11666975001 Roche, Mannheim, Germany Devices flow cytometer FACScalibur Becton Dickinson, Franklin Lakes, NJ, USA voltmeter Millicell-ERS Millipore, Burlington, MA, USA Pore Size:Article Title: Cyclophilins A and B oppositely regulate renal tubular epithelial cell phenotype Article Snippet: Materials Product Reference Supplier Cells HK-2 #CRL-2190 ATCC, Manassas, VA, USA RPTEC/TERT1 #CRL-4031 ATCC, Manassas, VA, USA Compounds TGFβ #100-B-001 R&D Systems, Minneapolis, MN, USA MG-132 #BML-PI102 Enzo Life Sciences, Farmingdale, NY, USA cyclosporine-A #239835 Calbiochem, San Diego, CA, USA brefeldin-A #B6542 Sigma-Aldrich, St. Louis, MO, USA human CypB #NBC1-18424 Novus Biologicals, Littleton, CO, USA ionomycin #I0634 Sigma-Aldrich, St. Louis, MO, USA thapsigargin #586005 Calbiochem, San Diego, CA, USA Plates and inserts 24-well transwell plates with 0.4 μm pore polyester membrane inserts (HTS transwell-24 PET) #CLS3379-2EA Sigma-Aldrich, St. Louis, MO, USA Polycarbonate cell culture inserts with 8 μm pore size and 0.47 cm2 growth area (1×105 pores/cm2) #140629 NuncTM-ThermoFisher, Waltham, MA, USA silicone culture inserts #81176 Ibidi GmbH, Martinsried, Germany growth factor reduced (GFR)MatrigelTM Basement Membrane Matrix #356230 BD Biosciences, Franklin Lakes, NJ, USA Kits Lipofectamine 3000 transfection kit #L3000-001 ThermoFischer, Waltham, MA, USA High-Capacity RNA-to-cDNA kit #4387406 Applied Biosystems, Foster City, CA, USA TRIzol Reagent #15596-026 Ambion-Life Technolog., Carlsbad, CA, USA QuikChange II XL Site-Directed Mutagenesis Kit #200522 Agilent Technologies, Santa Clara, CA, USA Cell Proliferation Kit II (XTT) #11465015001 Roche, Mannheim, Germany Dual-Luciferase Reporter Assay System #E1910 Promega, Fitchburg, WI, USA CoIP anti-HA Affinity Matrix, clone 3F10 #11815016001 Roche, Mannheim, Germany HA peptide #11666975001 Roche, Mannheim, Germany Devices flow cytometer FACScalibur Becton Dickinson, Franklin Lakes, NJ, USA voltmeter Millicell-ERS Millipore, Burlington, MA, USA Transfection:Article Title: Cyclophilins A and B oppositely regulate renal tubular epithelial cell phenotype Article Snippet: Materials Product Reference Supplier Cells HK-2 #CRL-2190 ATCC, Manassas, VA, USA RPTEC/TERT1 #CRL-4031 ATCC, Manassas, VA, USA Compounds TGFβ #100-B-001 R&D Systems, Minneapolis, MN, USA MG-132 #BML-PI102 Enzo Life Sciences, Farmingdale, NY, USA cyclosporine-A #239835 Calbiochem, San Diego, CA, USA brefeldin-A #B6542 Sigma-Aldrich, St. Louis, MO, USA human CypB #NBC1-18424 Novus Biologicals, Littleton, CO, USA ionomycin #I0634 Sigma-Aldrich, St. Louis, MO, USA thapsigargin #586005 Calbiochem, San Diego, CA, USA Plates and inserts 24-well transwell plates with 0.4 μm pore polyester membrane inserts (HTS transwell-24 PET) #CLS3379-2EA Sigma-Aldrich, St. Louis, MO, USA Polycarbonate cell culture inserts with 8 μm pore size and 0.47 cm2 growth area (1×105 pores/cm2) #140629 NuncTM-ThermoFisher, Waltham, MA, USA silicone culture inserts #81176 Ibidi GmbH, Martinsried, Germany growth factor reduced (GFR)MatrigelTM Basement Membrane Matrix #356230 BD Biosciences, Franklin Lakes, NJ, USA Kits Lipofectamine 3000 transfection kit #L3000-001 ThermoFischer, Waltham, MA, USA High-Capacity RNA-to-cDNA kit #4387406 Applied Biosystems, Foster City, CA, USA TRIzol Reagent #15596-026 Ambion-Life Technolog., Carlsbad, CA, USA QuikChange II XL Site-Directed Mutagenesis Kit #200522 Agilent Technologies, Santa Clara, CA, USA Cell Proliferation Kit II (XTT) #11465015001 Roche, Mannheim, Germany Dual-Luciferase Reporter Assay System #E1910 Promega, Fitchburg, WI, USA CoIP anti-HA Affinity Matrix, clone 3F10 #11815016001 Roche, Mannheim, Germany HA peptide #11666975001 Roche, Mannheim, Germany Devices flow cytometer FACScalibur Becton Dickinson, Franklin Lakes, NJ, USA voltmeter Millicell-ERS Millipore, Burlington, MA, USA Mutagenesis:Article Title: Cyclophilins A and B oppositely regulate renal tubular epithelial cell phenotype Article Snippet: Materials Product Reference Supplier Cells HK-2 #CRL-2190 ATCC, Manassas, VA, USA RPTEC/TERT1 #CRL-4031 ATCC, Manassas, VA, USA Compounds TGFβ #100-B-001 R&D Systems, Minneapolis, MN, USA MG-132 #BML-PI102 Enzo Life Sciences, Farmingdale, NY, USA cyclosporine-A #239835 Calbiochem, San Diego, CA, USA brefeldin-A #B6542 Sigma-Aldrich, St. Louis, MO, USA human CypB #NBC1-18424 Novus Biologicals, Littleton, CO, USA ionomycin #I0634 Sigma-Aldrich, St. Louis, MO, USA thapsigargin #586005 Calbiochem, San Diego, CA, USA Plates and inserts 24-well transwell plates with 0.4 μm pore polyester membrane inserts (HTS transwell-24 PET) #CLS3379-2EA Sigma-Aldrich, St. Louis, MO, USA Polycarbonate cell culture inserts with 8 μm pore size and 0.47 cm2 growth area (1×105 pores/cm2) #140629 NuncTM-ThermoFisher, Waltham, MA, USA silicone culture inserts #81176 Ibidi GmbH, Martinsried, Germany growth factor reduced (GFR)MatrigelTM Basement Membrane Matrix #356230 BD Biosciences, Franklin Lakes, NJ, USA Kits Lipofectamine 3000 transfection kit #L3000-001 ThermoFischer, Waltham, MA, USA High-Capacity RNA-to-cDNA kit #4387406 Applied Biosystems, Foster City, CA, USA TRIzol Reagent #15596-026 Ambion-Life Technolog., Carlsbad, CA, USA QuikChange II XL Site-Directed Mutagenesis Kit #200522 Agilent Technologies, Santa Clara, CA, USA Cell Proliferation Kit II (XTT) #11465015001 Roche, Mannheim, Germany Dual-Luciferase Reporter Assay System #E1910 Promega, Fitchburg, WI, USA CoIP anti-HA Affinity Matrix, clone 3F10 #11815016001 Roche, Mannheim, Germany HA peptide #11666975001 Roche, Mannheim, Germany Devices flow cytometer FACScalibur Becton Dickinson, Franklin Lakes, NJ, USA voltmeter Millicell-ERS Millipore, Burlington, MA, USA Reporter Assay:Article Title: Cyclophilins A and B oppositely regulate renal tubular epithelial cell phenotype Article Snippet: Materials Product Reference Supplier Cells HK-2 #CRL-2190 ATCC, Manassas, VA, USA RPTEC/TERT1 #CRL-4031 ATCC, Manassas, VA, USA Compounds TGFβ #100-B-001 R&D Systems, Minneapolis, MN, USA MG-132 #BML-PI102 Enzo Life Sciences, Farmingdale, NY, USA cyclosporine-A #239835 Calbiochem, San Diego, CA, USA brefeldin-A #B6542 Sigma-Aldrich, St. Louis, MO, USA human CypB #NBC1-18424 Novus Biologicals, Littleton, CO, USA ionomycin #I0634 Sigma-Aldrich, St. Louis, MO, USA thapsigargin #586005 Calbiochem, San Diego, CA, USA Plates and inserts 24-well transwell plates with 0.4 μm pore polyester membrane inserts (HTS transwell-24 PET) #CLS3379-2EA Sigma-Aldrich, St. Louis, MO, USA Polycarbonate cell culture inserts with 8 μm pore size and 0.47 cm2 growth area (1×105 pores/cm2) #140629 NuncTM-ThermoFisher, Waltham, MA, USA silicone culture inserts #81176 Ibidi GmbH, Martinsried, Germany growth factor reduced (GFR)MatrigelTM Basement Membrane Matrix #356230 BD Biosciences, Franklin Lakes, NJ, USA Kits Lipofectamine 3000 transfection kit #L3000-001 ThermoFischer, Waltham, MA, USA High-Capacity RNA-to-cDNA kit #4387406 Applied Biosystems, Foster City, CA, USA TRIzol Reagent #15596-026 Ambion-Life Technolog., Carlsbad, CA, USA QuikChange II XL Site-Directed Mutagenesis Kit #200522 Agilent Technologies, Santa Clara, CA, USA Cell Proliferation Kit II (XTT) #11465015001 Roche, Mannheim, Germany Dual-Luciferase Reporter Assay System #E1910 Promega, Fitchburg, WI, USA CoIP anti-HA Affinity Matrix, clone 3F10 #11815016001 Roche, Mannheim, Germany HA peptide #11666975001 Roche, Mannheim, Germany Devices flow cytometer FACScalibur Becton Dickinson, Franklin Lakes, NJ, USA voltmeter Millicell-ERS Millipore, Burlington, MA, USA Co-Immunoprecipitation Assay:Article Title: Cyclophilins A and B oppositely regulate renal tubular epithelial cell phenotype Article Snippet: Materials Product Reference Supplier Cells HK-2 #CRL-2190 ATCC, Manassas, VA, USA RPTEC/TERT1 #CRL-4031 ATCC, Manassas, VA, USA Compounds TGFβ #100-B-001 R&D Systems, Minneapolis, MN, USA MG-132 #BML-PI102 Enzo Life Sciences, Farmingdale, NY, USA cyclosporine-A #239835 Calbiochem, San Diego, CA, USA brefeldin-A #B6542 Sigma-Aldrich, St. Louis, MO, USA human CypB #NBC1-18424 Novus Biologicals, Littleton, CO, USA ionomycin #I0634 Sigma-Aldrich, St. Louis, MO, USA thapsigargin #586005 Calbiochem, San Diego, CA, USA Plates and inserts 24-well transwell plates with 0.4 μm pore polyester membrane inserts (HTS transwell-24 PET) #CLS3379-2EA Sigma-Aldrich, St. Louis, MO, USA Polycarbonate cell culture inserts with 8 μm pore size and 0.47 cm2 growth area (1×105 pores/cm2) #140629 NuncTM-ThermoFisher, Waltham, MA, USA silicone culture inserts #81176 Ibidi GmbH, Martinsried, Germany growth factor reduced (GFR)MatrigelTM Basement Membrane Matrix #356230 BD Biosciences, Franklin Lakes, NJ, USA Kits Lipofectamine 3000 transfection kit #L3000-001 ThermoFischer, Waltham, MA, USA High-Capacity RNA-to-cDNA kit #4387406 Applied Biosystems, Foster City, CA, USA TRIzol Reagent #15596-026 Ambion-Life Technolog., Carlsbad, CA, USA QuikChange II XL Site-Directed Mutagenesis Kit #200522 Agilent Technologies, Santa Clara, CA, USA Cell Proliferation Kit II (XTT) #11465015001 Roche, Mannheim, Germany Dual-Luciferase Reporter Assay System #E1910 Promega, Fitchburg, WI, USA CoIP anti-HA Affinity Matrix, clone 3F10 #11815016001 Roche, Mannheim, Germany HA peptide #11666975001 Roche, Mannheim, Germany Devices flow cytometer FACScalibur Becton Dickinson, Franklin Lakes, NJ, USA voltmeter Millicell-ERS Millipore, Burlington, MA, USA Flow Cytometry:Article Title: Cyclophilins A and B oppositely regulate renal tubular epithelial cell phenotype Article Snippet: Materials Product Reference Supplier Cells HK-2 #CRL-2190 ATCC, Manassas, VA, USA RPTEC/TERT1 #CRL-4031 ATCC, Manassas, VA, USA Compounds TGFβ #100-B-001 R&D Systems, Minneapolis, MN, USA MG-132 #BML-PI102 Enzo Life Sciences, Farmingdale, NY, USA cyclosporine-A #239835 Calbiochem, San Diego, CA, USA brefeldin-A #B6542 Sigma-Aldrich, St. Louis, MO, USA human CypB #NBC1-18424 Novus Biologicals, Littleton, CO, USA ionomycin #I0634 Sigma-Aldrich, St. Louis, MO, USA thapsigargin #586005 Calbiochem, San Diego, CA, USA Plates and inserts 24-well transwell plates with 0.4 μm pore polyester membrane inserts (HTS transwell-24 PET) #CLS3379-2EA Sigma-Aldrich, St. Louis, MO, USA Polycarbonate cell culture inserts with 8 μm pore size and 0.47 cm2 growth area (1×105 pores/cm2) #140629 NuncTM-ThermoFisher, Waltham, MA, USA silicone culture inserts #81176 Ibidi GmbH, Martinsried, Germany growth factor reduced (GFR)MatrigelTM Basement Membrane Matrix #356230 BD Biosciences, Franklin Lakes, NJ, USA Kits Lipofectamine 3000 transfection kit #L3000-001 ThermoFischer, Waltham, MA, USA High-Capacity RNA-to-cDNA kit #4387406 Applied Biosystems, Foster City, CA, USA TRIzol Reagent #15596-026 Ambion-Life Technolog., Carlsbad, CA, USA QuikChange II XL Site-Directed Mutagenesis Kit #200522 Agilent Technologies, Santa Clara, CA, USA Cell Proliferation Kit II (XTT) #11465015001 Roche, Mannheim, Germany Dual-Luciferase Reporter Assay System #E1910 Promega, Fitchburg, WI, USA CoIP anti-HA Affinity Matrix, clone 3F10 #11815016001 Roche, Mannheim, Germany HA peptide #11666975001 Roche, Mannheim, Germany Devices flow cytometer FACScalibur Becton Dickinson, Franklin Lakes, NJ, USA voltmeter Millicell-ERS Millipore, Burlington, MA, USA shRNA:Article Title: Cyclophilins A and B oppositely regulate renal tubular epithelial cell phenotype Article Snippet: Materials Product Reference Supplier Cells HK-2 #CRL-2190 ATCC, Manassas, VA, USA RPTEC/TERT1 #CRL-4031 ATCC, Manassas, VA, USA Compounds TGFβ #100-B-001 R&D Systems, Minneapolis, MN, USA MG-132 #BML-PI102 Enzo Life Sciences, Farmingdale, NY, USA cyclosporine-A #239835 Calbiochem, San Diego, CA, USA brefeldin-A #B6542 Sigma-Aldrich, St. Louis, MO, USA human CypB #NBC1-18424 Novus Biologicals, Littleton, CO, USA ionomycin #I0634 Sigma-Aldrich, St. Louis, MO, USA thapsigargin #586005 Calbiochem, San Diego, CA, USA Plates and inserts 24-well transwell plates with 0.4 μm pore polyester membrane inserts (HTS transwell-24 PET) #CLS3379-2EA Sigma-Aldrich, St. Louis, MO, USA Polycarbonate cell culture inserts with 8 μm pore size and 0.47 cm2 growth area (1×105 pores/cm2) #140629 NuncTM-ThermoFisher, Waltham, MA, USA silicone culture inserts #81176 Ibidi GmbH, Martinsried, Germany growth factor reduced (GFR)MatrigelTM Basement Membrane Matrix #356230 BD Biosciences, Franklin Lakes, NJ, USA Kits Lipofectamine 3000 transfection kit #L3000-001 ThermoFischer, Waltham, MA, USA High-Capacity RNA-to-cDNA kit #4387406 Applied Biosystems, Foster City, CA, USA TRIzol Reagent #15596-026 Ambion-Life Technolog., Carlsbad, CA, USA QuikChange II XL Site-Directed Mutagenesis Kit #200522 Agilent Technologies, Santa Clara, CA, USA Cell Proliferation Kit II (XTT) #11465015001 Roche, Mannheim, Germany Dual-Luciferase Reporter Assay System #E1910 Promega, Fitchburg, WI, USA CoIP anti-HA Affinity Matrix, clone 3F10 #11815016001 Roche, Mannheim, Germany HA peptide #11666975001 Roche, Mannheim, Germany Devices flow cytometer FACScalibur Becton Dickinson, Franklin Lakes, NJ, USA voltmeter Millicell-ERS Millipore, Burlington, MA, USA Sequencing:Article Title: Cyclophilins A and B oppositely regulate renal tubular epithelial cell phenotype Article Snippet: Materials Product Reference Supplier Cells HK-2 #CRL-2190 ATCC, Manassas, VA, USA RPTEC/TERT1 #CRL-4031 ATCC, Manassas, VA, USA Compounds TGFβ #100-B-001 R&D Systems, Minneapolis, MN, USA MG-132 #BML-PI102 Enzo Life Sciences, Farmingdale, NY, USA cyclosporine-A #239835 Calbiochem, San Diego, CA, USA brefeldin-A #B6542 Sigma-Aldrich, St. Louis, MO, USA human CypB #NBC1-18424 Novus Biologicals, Littleton, CO, USA ionomycin #I0634 Sigma-Aldrich, St. Louis, MO, USA thapsigargin #586005 Calbiochem, San Diego, CA, USA Plates and inserts 24-well transwell plates with 0.4 μm pore polyester membrane inserts (HTS transwell-24 PET) #CLS3379-2EA Sigma-Aldrich, St. Louis, MO, USA Polycarbonate cell culture inserts with 8 μm pore size and 0.47 cm2 growth area (1×105 pores/cm2) #140629 NuncTM-ThermoFisher, Waltham, MA, USA silicone culture inserts #81176 Ibidi GmbH, Martinsried, Germany growth factor reduced (GFR)MatrigelTM Basement Membrane Matrix #356230 BD Biosciences, Franklin Lakes, NJ, USA Kits Lipofectamine 3000 transfection kit #L3000-001 ThermoFischer, Waltham, MA, USA High-Capacity RNA-to-cDNA kit #4387406 Applied Biosystems, Foster City, CA, USA TRIzol Reagent #15596-026 Ambion-Life Technolog., Carlsbad, CA, USA QuikChange II XL Site-Directed Mutagenesis Kit #200522 Agilent Technologies, Santa Clara, CA, USA Cell Proliferation Kit II (XTT) #11465015001 Roche, Mannheim, Germany Dual-Luciferase Reporter Assay System #E1910 Promega, Fitchburg, WI, USA CoIP anti-HA Affinity Matrix, clone 3F10 #11815016001 Roche, Mannheim, Germany HA peptide #11666975001 Roche, Mannheim, Germany Devices flow cytometer FACScalibur Becton Dickinson, Franklin Lakes, NJ, USA voltmeter Millicell-ERS Millipore, Burlington, MA, USA |